Role of Catha Edulis (Khat) in Free Radicals Formation in Vivo and in Vitro Study

  • Mohammed S.


The Khat Catha edulis Celastraceae chewing resulted in many diseases and physiological alterations affecting man. This fact has been observed in several previous studies. The present work was evaluated for the first time to investigate the in vivo and in vitro mode of Khat action and how it causing the etiology of diseases. Thirty healthy male rabbits weighting 1600-1800gm. were divided into 3 groups with 10 animals in each first group served as control animals they received orally10 ml.of normal saline daily period of 30 day while animals in second and third groups received a single orally dose 5g/kg and 10g/kg respectively of powdered khat leaves dissolved in distilled water period of 30 days. The level of lipid peroxidation LPO and Methylglyoxal MG in liver homogenate were measured and the levels of hydroxyl and superoxide radicals generation in the in vitro reactions with presence of khat were also measured. Results showed a dose dependent high significant P0.01 increase in the level of LPO MG in liver homogenate of animals treated with Khat as compared to control animals. Results of in vitro reactions showed increase in the superoxide and hydroxyl radicals generation.